Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs) 2018
December 10 - 14, 2018
Hinxton , 英国
div>After attending this course, participants should be able to:
Recognise key principles of GCCP and aseptic processing including the role of antibiotics
Assess and identify key advantages, disadvantages and critical elements of different approaches to somatic cell reprogramming
Assess and identify undifferentiated, fully reprogrammed iPSCs compared to partially reprogrammed iPSC colonies
Recognise critical aspects for successful preservation and storage
Evaluate the recovery of iPSCs and identify viable cells
Identify undifferentiated morphology of confluent iPSCs ready for passaging
Identify and troubleshoot critical aspects in the passaging and maintenance of undifferentiated iPSCs
Recognise the importance of core QC and potential consequences of failing to carry it out
Evaluate different capabilities of each method of determining potential pluripotency
Understand the basic principles of flow cytometry and its application to iPSC
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Derivation and Culture of Human Induced Pluripotent Stem Cells (hiPSCs) December 10 - 14, 2018
