Difficult to Express Proteins 2014

Cambridge Healthtech Institute’s Ninth Annual

Difficult to Express Proteins

Harnessing Innovation to Improve Expression and Function

Part of the Tenth Annual PEGS: the essential protein engineering summit

May 5-6, 2014 | Seaport World Trade Center | Boston, MA

Membrane proteins, ion channels, toxic or insoluble proteins, low abundance protein complexes, vaccines and other “finicky” proteins often are the best choices for therapeutics, yet their successful expression is a minefield of failures and challenges. This conference will present innovative and imaginative methods to make those difficult to express proteins more “expressible” and functional.

Topics will include, but are not limited to:

Novel host, vector, transfection and cloning strategies

Solving problems in recombinant protein expression

Cell-free methods for finicky proteins

Controlling disulfide bonds

Folding, refolding and function

Improving expression of extra-cellular proteins

Rational vector design and pathway modulation to facilitate expression of difficult proteins

Utilizing transient gene expression

Achieving proper glycosylation, pegylation, sialylation, etc.

Expression of protein complexes and toxic proteins

Overcoming ion channel expression problems